The CKX53 inverted microscope is designed for routine cell culture observation, cell sampling and image capture. Its configuration can be selected for brightfield, phase contrast, inversion contrast (IVC) and fluorescence observation, according to the cell culture workflow, sample container and documentation requirements.
CKX53 Configuration Overview
The CKX53 uses a UIS2 optical system with a long-life 4000 K LED light source. It has a fixed trinocular observation tube with a 45-degree inclination, a 10x eyepiece and a field number of 22. The built-in camera port can be used with a compatible camera adapter and C-mount camera for image documentation.
The standard plain stage measures 200 mm x 125 mm. A mechanical stage is available as an option for controlled movement and microplate observation. The selected holder and stage configuration should be matched to the culture vessel, such as dishes, microplates, flasks or multi-layer tissue flasks.
Select the Observation Method You Need
Brightfield Observation
The brightfield entry configuration uses 4x and 10x objectives for routine observation of stained samples, including applications such as plant material, plankton and similar specimens.
Phase Contrast Observation
Phase contrast configurations are available for observing the condition and activity of transparent live cells. Depending on the configuration, phase contrast objectives can cover 4x, 10x, 20x and 40x observation. An integrated phase contrast slider helps support efficient observation without changing the ring slit when switching between compatible objective magnifications.
Inversion Contrast (IVC) Observation
The CKX53 can be configured with the inversion contrast technique for clear three-dimensional views of transparent objects. This configuration is intended for applications that benefit from reduced halos and directional shadows. The compatible objectives and slider components should be selected for the required observation method.
Fluorescence Observation
A fluorescence configuration can be selected for fluorescence observation. The configuration uses compatible fluorescence components, including a 100 W mercury lamp housing, fluorescence mirror units and applicable filters. Fluorescence dye, filter and imaging requirements should be confirmed before quotation.
Designed for Cell Culture Containers
The CKX53 supports a range of cell culture containers through compatible holders. A universal holder can be used with dishes, microplates and flasks. With an optional holder, up to three 35 mm dishes can be placed on the stage. For 96-well plate observation, the mechanical stage can help identify and move between well positions.
The system can also be configured for multi-layer tissue flasks. When the condenser is detached and a compatible objective is selected, containers up to 190 mm in height can be observed and the lower layers of a multi-layer tissue flask can be accessed.
Image Capture and Daily Workflow
The CKX53 is built for routine observation in a clean bench environment. Its compact design, approximately 7 kg weight and ergonomic placement of the observation tube and controls help support comfortable cell sampling and handling. Full LED illumination is available when the system is switched on, helping users begin routine observation promptly.
For documentation, use the integrated camera port with a compatible camera adapter and camera. The final camera, software and image capture configuration should be selected according to the required workflow; optional accessories are not included by default.
Configure the CKX53 for Your Workflow
Before requesting a quotation, please prepare the following information:
- Cell type, culture vessel and sample preparation method
- Required observation method: brightfield, phase contrast, inversion contrast or fluorescence
- Required objective magnifications
- Whether a mechanical stage, microplate handling or multi-layer flask observation is required
- Whether camera documentation, fluorescence filters or other accessories are needed
This information helps Cuihe recommend a compatible CKX53 configuration and suitable accessories for your application.
Request a CKX53 Configuration
The CKX53 is a configurable inverted microscope system rather than a single fixed specification. Contact Cuihe with your cell culture application, container type and observation requirements to discuss a suitable configuration.
Related Reading
For help selecting an inverted microscope for cell culture, read our guide: How to Choose an Inverted Microscope for Cell Culture.
To compare CKX53 and TS2 configurations, read: CKX53 vs TS2 Inverted Microscope.